We tested three melanoma cell lines, primary (VGP) WM3211 and metastatic: Me15392 and MEWO, for 25μM concentrations of chloroquine and mTOR inhibitor everolimus in concentrations ranging from 20 nM to 25 μM and 50 μM concentrations of rapamycin alone and in combination with chloroquine. This evidence concerns the gene MTOR and melanoma.