Seeing as the accumulation of aberrant cholesterol within the LE/Lys compartment and the consequential juxtanuclear clustering of lysosomes is an important cellular hallmark of NPC disease [18,19], lysosomal positioning was surveyed in the NPC1−/− ARPE-19 cell lines transduced with the different variants (and variant combinations) of NPC1. Here, NPC1 is linked to nasopharyngeal carcinoma.