Furthermore, α-mangostin (10, 20, 30, 40, and 50 μM) demonstrated suppression of the Wnt/β-catenin pathway in osteosarcoma cells by reducing the protein levels of Wnt3a, phosphorylated glycogen synthase kinase 3 beta (GSK3β), and nuclear β-catenin [25]. The gene discussed is GSK3B; the disease is osteosarcoma.