RHOA and glaucoma: In addition, a previous study using a rat model with elevated IOP showed that Rap caused IOP reduction through an increase of the AH outflow facility, presumably via regulation of the RhoA-related actin cytoskeleton in TM cells, and also protected retinal ganglion cells by inhibiting the activation of glial cells and the release of proinflammatory factors, suggesting that targeting Rap may be an effective strategy for the treatment of glaucoma [43].