Building on our previous studies demonstrating the therapeutic efficacy of an autologous leucoconcentrate enriched with three recombinant genes encoding VEGF165, GDNF, and NCAM1, using Ad5/35F, in treating mini-pigs with ischemic stroke [35,36] and spinal cord injury [37] we now report the biosafety evaluation of this chimeric adenoviral vector in vitro and in vivo studies in mini-pigs. The gene discussed is GDNF; the disease is ischemic stroke.