Furthermore, m5C-Bis-Seq and mRNA-Seq analyses identified LAMC2 as a potential target regulated by NSUN2 in promoting EMT in HNSCC, providing novel insights into how NSUN2 modulates the m5C landscape and facilitates cancer progression through the stabilization of LAMC2 mRNA. This evidence concerns the gene LAMC2 and head and neck squamous cell carcinoma.