NSUN2 silencing attenuates EMT signal transduction by reducing m5C modification levels in LAMC2 mRNA, while LAMC2 overexpression reverses the inhibitory effect on the malignant cell phenotype in NSUN2-depleted cells, highlighting LAMC2 as a key mediator induced by NSUN2 in HNSCC. The gene discussed is NSUN2; the disease is head and neck squamous cell carcinoma.