To further elucidate the mechanism of SAR1A in HNSCC carcinogenesis, we confirmed a reduction in mRNA levels of SAR1A in SAR1A-sh1 and SAR1A-sh2 HNSCC cells (Figure 6A), which was followed by transcriptome sequencing of Cal-27 cells from SAR1A knockdown and control groups. The gene discussed is SAR1A; the disease is head and neck squamous cell carcinoma.