BGLAP and neoplasm: Finally, ELISA assay can be performed on serum collected from animal at the time of sacrifice, to check for the presence of bone turnover markers, such as carboxy-terminal cross-linking telopeptide of type 1 collagen (CTX), TRAcP, bone alkaline phosphatase (BALP), and osteocalcin (OCN), hormones, inflammatory or tumor-derived growth factors [111].