Upregulated proteins, such as nucleolin (NCL), galectin-3-binding protein (LGALS3BP) and neural cell adhesion molecule (NCAM), and the downregulated proteins, such as lumican (LUM), vasodilator stimulated phosphoprotein (VASP) and decorin (DCN) were found to be able to discriminate the NB patients from controls; meanwhile, up-regulation of myosin-9 (MYH9), fibronectin (FN1) and latent-transforming growth factor-beta-binding protein 1 (LTBP1), and down-regulation of calreticulin (CALR) and A-kinase anchor protein 12 (AKAP12) were also identified in HR-NB patients as compared to LR-NB patients. The gene discussed is NCAM1; the disease is neuroblastoma.