For instance, Tsutsumi et al. showed that exposing the B16 melanoma cells to DMAG-N-oxide, a cell-impermeant N-terminal inhibitor of Hsp90, prior to injecting them into mice resulted in a reduced metastatic focus within the lungs, when compared to metastatic deposits in the mice injected with untreated B16 cells [32]. This evidence concerns the gene HSP90AA1 and melanoma.