In alignment with previous research on the influence of AGK on cell apoptosis [22], silencing the AGK gene using siRNA induced apoptosis in breast cancer cells (Figure S1E), and Annexin V/PI staining confirmed that concentrations of 10 μmol/L and 15 μmol/L of Netupitant induced apoptosis in breast cancer cells in a dose-dependent manner (Figure 2C). This evidence concerns the gene AGK and breast cancer.