Recombinant TIMP-1 conjugated to glycosylphosphatidylinositol (TIMP-1-GPI) when combined with sublethal hyperthermic treatment demonstrated efficacy against melanoma cell lines 624.38-MEL, 93.04A12MEL, SK-MEL23, WM115, and WM266-4 under in vitro conditions [145]. The gene discussed is TIMP1; the disease is melanoma.