mRNA and protein expression of TSLP is upregulated in the skin lesions from patients with AD in association with promoter hypomethylation (detected by using bisulfite sequencing), suggesting that DNA demethylation (detected by using 5-aza, a DNA methyltransferase inhibitor) of a regulatory region of TSLP may contribute to its overexpression in AD skin lesions [102]. The gene discussed is TSLP; the disease is Alzheimer disease.