In tumor sphere formation assays, CIITA-spheres (of two separate clones) were also reproducibly smaller and unevenly formed after 7 days, as compared to wildtype spheres (GL261-CIITA-C1 33.1 ± 6.6 μm and GL261-CIITA-C2 35.4 ± 9.2 μm versus GL261-WT 71.2 ± 15.2 μm) (p < 0.0001, one-way ANOVA, Figure 2C,D). Here, CIITA is linked to neoplasm.