To study the underlying molecular mechanism of the anti-obesity effect of ABS in adipocytes, we also conducted the immunofluorescence analysis of β-catenin, C/EBPα, and PPARγ in the 3L3-L1 cell line (Figure 14A,B and Figure 15A–D). This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.