This procedure revealed that (i) malignant and non-malignant cells clustered differently, (ii) there was a clear proteomic difference between AR-positive (22Rv1 and LNCaP) and AR-negative (PC3) PCa cells, (iii) AR-positive PCa cells exhibited similar clustering patterns, and (iv) switching the medium from KSFM to MEM had only a minor impact on the proteome of RWPE-1 cells. This evidence concerns the gene AR and posterior cortical atrophy.