Numerous experiments were conducted to investigate the impact of 1 × 109 CFU/mL B. longum BLG1 and 1 × 109 CFU/mL B. psychaerophilum Q5, both individually and in combination, on an in vitro co-culture prostatic model of BPH under hyperplasia conditions induced by the pre-treatment with 10 nM DHT for 4 days, as ROS production and interleukins activation, including TNF-α, IL6, and IL10, were critical factors in the development of BPH. The gene discussed is TNF; the disease is benign prostatic hyperplasia.