The ROC curves analysis demonstrated that HMGA1/B2/N1/N4 effectively distinguish HCC from normal samples, yielding AUC values of HMGA1(AUC = 0.913), HMGB2(AUC = 0.939), HMGN1(AUC = 0.905), HMGN4(AUC = 0.904). This evidence concerns the gene HMGB2 and hepatocellular carcinoma.