Furthermore, PCR and Western blot methods were used to detect the mRNA expression and protein levels of the predicted target genes of miR-130b, including PPAR-γ, PGC1α, PPARα, LDLR, SCD-1 and GR, and the results showed that although the mRNA expression of PGC1α was increased and the mRNA expression of PPARα was decreased, the mRNA expression of other genes and the protein levels of all above genes were not obviously changed (Figure 2B–E) in the HF-130b-MV-injected group compared with the HF-SC-MV group. This evidence concerns the gene PPARA and hydrops fetalis.