We found that the transcription factors of miR-101-3p target genes including AXIN, CK1, and GSK3, participated in the regulation of the Wnt signaling pathway, which may indicate that these are miR-101-3p hub target genes that may help us understand the regulation mechanisms in ESCC progression (Figure 4D). Here, AXIN1 is linked to esophageal squamous cell carcinoma.