Therefore, to determine the role of the PTPN6 gene in MDS, we knocked down PTPN6 expression in the SKM-1 cell line using lentiviral transduction, with pGMLV-SC5 RNAi as the vector and H_PTPN6-shRNA1149 selected to verify the effect of PTPN6 knockdown. Here, PTPN6 is linked to myelodysplastic syndrome.