Interestingly, we noted a significant downregulation of the expression of multiple coinhibitory molecules associated with CAR-T cell exhaustion (37), including LAG-3, PD-1, and TIM-3, in ITK-KO CD19-CAR-T cells compared with nt-KO CD19-CAR-T cells 48 hours after coculture with tumor cells (Supplemental Figure 4, A–D). Here, ITK is linked to neoplasm.