In addition, we also identified decreased levels of BDNF, in SOD1-ALS MNs, which is a key growth factor required for the cell growth/neuronal survival in the brain, whereas reduced expression has been associated with neuronal dysfunction and death in several neurological disorders including ALS (Alvia et al., 2022; Lanuza et al., 2019). This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.