NFE2L2 and Alzheimer disease: To examine the presence of potential age-related sex differences on transcriptional regulation during normal aging and AD, immunoblots of several key transcription factor proteins or their subunits (NF-κB p50, p105, p65, pIκB-α, and Nrf2) implicated in neuroinflammatory signaling were measured in isolated hypothalamic mitochondria from 3xTg female and male mice vs. their respective controls at 2, 6, and 13 months of age (Fig. 5A-E).