Wound healing and Transwell assays were performed to examine the influence on CRC cells’ migration and invasion abilities, and the results showed that knockdown of PCDHGA9 led to a faster scratch healing rate among CRC cells and an increased number of cells invading through the Transwell membrane compared with the negative control (NC) groups, while opposite results were observed in the overexpression of PCDHGA9 groups (Fig. 2a, b). This evidence concerns the gene PCDHGA9 and colorectal carcinoma.