Alongside a validated and previously published Zfp189-targeting sgRNA [9, 11], we directed CRISPR-mediated Zfp189 activation, with dCas9-VP64, or CRISPR-mediated repression, with dCas9-G9a, in mouse neuroblastoma Neuro-2a (N2a) cells (Fig. 1B). This evidence concerns the gene EHMT2 and neuroblastoma.