NKX2-1 and adenocarcinoma: Through pseudotime analysis and the expression level of Nkx2-1 (Figs. S2D–F), we can infer that cluster 2 represents the initial adenocarcinoma cells, while clusters 0 and 1 are mucinous cells formed after Nkx2-1 knockout, and cluster 3 represents an intermediate state of LUAD transitioning towards IMA (Fig. 1C).