The initial tumor formation took a long time (more than 6 months), but thereafter passage took 2–3 months. All transplanted FLC organoids (100%) formed tumors even at very low inoculum concentrations (<100 cells). However, the tumorigenesis rate depended on the number of organoids transplanted; was increased by dietary supplementation (e.g. HGF and VEGF); and by transplantation of organoids embedded in hyaluronan hydrogels. This evidence concerns the gene VEGFA and neoplasm.