A total of 9691, 10967, 10016, 9969 and 1076928 DEGs were associated with the pathway of “Metabolic pathways (map01100)” in CF/CK,NF25/CK, NF50/CK, NF75/CK and NF100/CK, respectively. The gene discussed is CYP3A4; the disease is cystic fibrosis.