To further investigate the direct effect of Prrc2a on cancer cell stemness, we induced CSC‐rich spheroids grown from a single HCT116 cell.[33] Knockdown of PRRC2A inhibited the formation of HCT116 tumor spheroids, as quantified by the percentage of spheroids that had diameters larger than 75 μm (Figure 2H,I). The gene discussed is PRRC2A; the disease is cancer.