The BioCarta signaling pathways enriched in TFAM-deficient colorectal cancer cells included G1/S check point regulation, selective expression of chemokine receptors during T-cell polarization (Natural killer T (NKT) cells), transcription factor CRE-binding protein (CREB) signaling, signaling pathway from G-protein families, cytokines and inflammatory response, sonic hedgehog (shh) signaling, and M-calpain pathway (Fig. 4A, B). The gene discussed is SHH; the disease is colorectal cancer.