To test if Oct1 or Oct2 are important for Dnmt3a/Flt3-mutant AML self-renewal and/or proliferation and if miR196b–/–Dnmt3a+/–Flt3ITD AML is preferentially sensitive to the levels of these transcription factors, we expressed two independent shRNA against Oct1 and Oct2 in Dnmt3a+/–Flt3ITD and miR196b–/–Dnmt3a+/–Flt3ITD AML. Here, POU2F1 is linked to acute myeloid leukemia.