Relative to non-targeting (NTsh) control shRNA, Oct1 and Oct2 knockdown significantly reduced the colony forming potential of miR196b–/–Dnmt3a+/–Flt3ITD AML, but not Dnmt3a+/–Flt3ITD AML (Fig. 2G, H and Supplementary Fig. 2F, G), suggesting that the deletion of miR-196b creates a unique requirement for Oct1 and Oct2 in miR196b-/-Dnmt3a+/-Flt3ITD AML. This evidence concerns the gene POU2F1 and acute myeloid leukemia.