Concomitantly, an elevation of circulating TG and free fatty acids (FFA), alterations in the glucose tolerance test (GTT) and insulin tolerance tests (ITT) and signs of T2D such as hyperinsulinaemia and altered fasting plasma glucose, yielding increased homeostasis model assessment of insulin resistance (HOMA-IR) values, were found in KLH-only vaccinated mice treated with CORT, but not after KLH–ACBP vaccination (Fig. 2j and Supplementary Fig. 3). This evidence concerns the gene INS and type 2 diabetes mellitus.