DBI and Cushing syndrome due to macronodular adrenal hyperplasia: To demonstrate that ACBP/DBI is involved in Cushing’s syndrome, we used six different approaches for ACBP/DBI inhibition: (1) induction of ACBP/DBI neutralizing antibodies, (2) i.p. injection of a neutralizing mAb, (3) conditional whole-body or hepatocyte-specific knockout of the Dbi gene, (4) mutation of the ACBP/DBI receptor (Gabrg2F77I/F77I) and (5) treatment with T3 or (6) RES to block Dbi transcription.