Previous studies [19, 20, 23, 49, 69, 70] have highlighted the independent prognostic role of Ki67 and PR-ITH indicators in predicting clinical outcomes, including overall survival, BCSS, or disease-free survival in BC, often exceeding the prognostic value of biomarker expression levels or rates assessed by manual or digital IHC scoring techniques. The gene discussed is MKI67; the disease is breast cancer.