ESR1 and neoplasm: The computational method of Immunogradient, proposed by Rasmusson et al. [48], enabled precision sampling of the tumor-stroma interface zone (IZ) with quantification of spatial distribution profiles of immune cells across the IZ in several solid tumors, including early ER+ invasive breast carcinoma [48] and HER2-borderline (IHC 2+) BC [49].