NOX4 and obesity due to melanocortin 4 receptor deficiency: In rWAT, two-way ANOVA revealed statistically significant increase in p22 (p < 0.001), Nox2 (p < 0.001) and Nox4 (p < 0.01) expression due to obesity but administration of QCT did not significantly affect the expression of these genes (Table 3).