MYLK and colitis: The work have demonostrated that the increased intestinal tight junction permeability and disease severity as well as intestinal microbiome composition alteration were observed in two independent models of DSS-induced colitis after administration of long-term PPI in mice, which might be attributed to enhanced myosin light chain kinase (MLCK) activation and expression (Nighot et al., 2023; Son et al., 2022; Pai et al., 2023).