Interestingly, FAP+ αSMA+ subtypes were localized closer to tumor cells compared to the previously identified myCAFs (Figure 4E) (average minimal distance from the tumor cells: CAFs 9, 29.7 ± 25.5μm, n=2591 cells; CAFs 10, 36.6 ± 60.2μm, n=1437 cells; CAFs 11, 32.5 ± 36.9μm, n=1980 cells; vs. CAFs 2, 68.0 ± 63.3μm, n=5254 cells; CAFs 6, 95.7 ± 108.0μm, n=10905 cells; CAFs 15, 61.3 ± 79.7μm; n=4430 cells; p-value < 10-15 KS test for all conditions), as well as to the FAP+ αSMA- CAFs 14 subpopulation (56.4 ± 54.7μm, n= 1389; p-value < 10-15 KS test). The gene discussed is FAP; the disease is neoplasm.