CD4 and neoplasm: Analysis of the frequency of cells (Reference) in close proximity (<30μm) with other cell types (Target) showed a preferential neighboring of B cells to both CD4+ (72 ± 28% of B cells) and CD8+ T cells (81 ± 21% of B cells) whereas no interaction was observed between immune cells and PDAC, with the exception of a weak association with CD44+ macrophages (59 ± 24% of cells vs all the Tumor subtypes), M1-like macrophages (67 ± 20% of cells vs all the Tumor subtypes) and neutrophils (65 ± 25% of cells vs all tumor subtypes) (Figure 3G; Supplementary Figure 5).