Our results showed that there were lower levels of CD4 naïve T cells and CD4 memory activated T cells in IFNγ+CD3+ T cells induced by Mtb than in those induced by BCG and antigen (Figure 3E), These might indicate that antigen and BCG would induce more protective immune response than Mtb pathogen, since immune cell types and their quantities have been linked to TB outcomes, with differing infiltration of these cells in the lung microenvironment relating to Mtb survival (51–54). Here, CD4 is linked to tuberculosis.