Given the intricate role of CTSB, enolase, and GAL3 enzymes in lung cancer progression and the potential benefits of fisetin, this study aims to explore the effectiveness of a fisetin-loaded nano emulsion (FNE) in attenuating these pathological markers, in vitro, in lung cancer cells, specifically the A549 human lung adenocarcinoma cells. This evidence concerns the gene LGALS3 and lung carcinoma.