Previous studies have reported that activated HSCs mainly aggregate in regions such as tumor margins, the hepatic sinusoid, tumor stroma, and perivascular areas in HCC tissues.[14, 15] Consistently, our results demonstrated that miR‐500a‐3p exhibited a significant upregulation in these α‐SMA‐high expressing regions within tumor tissues (Figure S1, Supporting Information). Here, ACTA1 is linked to hepatocellular carcinoma.