Whereas MTHFD1 knockdown in HCT‐116 cells promoted Beclin1, ULK1 protein expression and the ratio of LC3‐II to LC3‐I, and down‐regulated P62 expression (Figure 4F–H), indicating that knockdown of MTHFD1 induced autophagy in CRC cells. This evidence concerns the gene BECN1 and colorectal carcinoma.