The qPCR targeting pagA with BAPA probe (pXO1) [3] and capC (pXO2) regions [3] used by Lekota et al.[42] has been reported to be inadequate for distinguishing closely related Bacillus species from anthrax outbreaks, while Zincke et al. [41] used capB, lef and Ba-1 targets to differentiate B. anthracis from B. cereus sensu stricto. This evidence concerns the gene PRDX1 and anthrax infection.