Multivariate Cox regression analysis confirmed that these 8 identified genes, including 2 protective elements (SQOR and PFKFB2) and 6 hazardous factors (MAFF, COL5A2, FAM162A, UQCRB, SFXN4, and COX6A2), could independently predict the overall survival of osteosarcoma samples (Figure 2E). Here, PFKFB2 is linked to osteosarcoma.