Furthermore, while we detected VIM and MSX2, previously reported mesenchymal hepatoblastoma markers22, as highly upregulated in the same cells that had a high embryonal-specific signature, we failed to detect other classical mesenchymal markers such as TWIST1, SNAI1, or SNAI2. Overall, this data suggested that the cultured cells primarily represented the epithelial components of primary hepatoblastomas, encompassing cells with both fetal and embryonal patterns of gene expression, the latter of which contains some mesenchymal features. Here, TWIST1 is linked to hepatoblastoma.