TWIST1 and neoplasm: The embryonal tumor cluster showed significant upregulation of key WNT signaling target genes and regulators, including APCDD128, NKD1, NOTUM, KREMEN1, TNFRSF19, and AXIN2, as well as WNT target genes that are associated with epithelial-to-mesenchymal transition (EMT) such as TWIST1, BMP4 and VIM29, along with low levels of hepatic markers compared to fetal tumors and normal hepatocytes (Fig. 1b, c and Supplementary Fig. 1c).