Then, HGP and gluconeogenesis obtained with GEMs were validated in an independent cohort, the “GNG cohort,” which included individuals with MASLD, with and without T2D, sharing characteristics similar to the “EPoS cohort” in terms of age, gender, weight, BMI, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) (Figure S6C). This evidence concerns the gene GPT and type 2 diabetes mellitus.