ACSL4-low-expressing (ACSL4-low) human HepG2, HCC-M, SNU-398 cell lines and rat N1S1 cell lines were sensitive to glucose- and glutamine-double starvation, while ACSL4-high-expressing (ACSL4-high) Huh7, Hep3B, and PLC/5 cells were not as affected (Fig. 2a-b). The gene discussed is ACSL4; the disease is hepatocellular carcinoma.