RAB7A and melanoma: Importantly, overexpression of MITF was able to increase proliferation in WT, confirming a direct effect of MITF on melanoma cell proliferation in SK-MEL-5, while both Rab7a and TPC2 KO lines did not show a difference in proliferation compared to empty vector expression after MITF OE, likely because MITF OE in the KOs can still not keep up with the continued degradation (Fig. S6d–h).