Further optimization of these compounds led to CT1113, it is a potent USP28 and USP25 inhibitor, experiments demonstrate that CT1113 can significantly increase the general level of USP25 substrate Tankyrase (TNKS) and inhibit the proliferation of various tumor cells, including Pancreatic Cancer Cancel LINE SW1990, Colon Cancer Cell Line HCT116 and lung cancer A549, etc., showing broad anti-tumor activity [101]. The gene discussed is TNKS; the disease is colonic neoplasm.