We performed additional IHC for PTEN and observed that men within the lower quartile of expression of loss of one tumor suppressor had shorter disease-free survival (PTEN low: HR 2.86, 95% CI 1.61–5.10, P = 0.0002; TTP low: HR 1.97, 95% CI 1.05–3.70, P = 0.03) (Figure 1D, left). This evidence concerns the gene PTEN and neoplasm.